The Biyamiti River rises in the south-western region of Kruger National Park near Pretoriuskop Camp. It flows eastwards until it joins the Crocodile River about 15 km west of Crocodile Bridge Camp.
The Biyamiti Bushveld Camp is situated on its banks along the S139 road. This road is reserved for residents of this camp.
The river does not flow all year round and it may be dry, or just a little stream, over winter.
The S23 road, which follows the river in parts, is quite scenic and is, at times, quite rewarding as far as game viewing goes.